![]() Proc Natl Acad Sci USA 27:499–506īerson SA, Yalow RS (1961) Immunochemical distinction between insulins with identical amino-acid sequences. Biochim Biophys Acta 608:19–31īeadle GW, Tatum EL (1941) Genetic control of biochemical reactions in Neurospora. Assembly of newly-synthesized large subunits into ribulose bisphosphate carboxylase in isolated intact pea chloroplasts. Nature 465:53–59īarraclough R, Ellis RJ (1980) Protein synthesis in chloroplasts. Ann Rev Biochem 44:453–475īarash J, Calarco JA, Gao W, Pan Q, Wang X, Shai O, Blencowe BJ, Frey BJ (2010) Deciphering the splicing code. J Exp Med 79:137–158īaldwin RL (1975) Intermediates in protein folding reactions and the mechanism of protein folding. Science 181:223–230Īvery OT, MacLeod CM, McCarthy M (1944) Studies on the chemical transformation of pneumococcal types. Biochem J 128:737–749Īnfinsen CB (1973) Principles that govern the folding of protein chains. Garland Publishing, New YorkĪnfinsen CB (1972) The formation and stabilisation of protein structure. A chemical chaperone just might be the difference between a failed experiment and a successful one.Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD (1994) Molecular biology of the cell, 3rd edn. The best chaperones are usually found experimentally, so I encourage you to experiment with them. What kind of chemical chaperone you choose is up to you. upregulation of endogenous folding systems using chemicals or heat, which in turn promote global protein folding.pharmacological chaperones, which are designed to promote the folding of a specific protein or to lock them into a stable form.In addition to chemical chaperones, there are several other methods and manipulations to promote protein folding. ![]() So bear this in mind if you do decide to use a chemical chaperone. Some argue that the use of chemical chaperones alters native protein structure, thereby compromising the biologic relevancy of your experiment. ![]() So, as with anything, there is a downside to using chemical chaperone. When You Should NOT Use Chemical Chaperones
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |